Direct localization of a β-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase
To identify the location of a domain of the β-subunit of Escherichia coli RNA polymerase (RNAP) on the three-dimensional structure, we developed a method to tag a nonessential surface of the multisubunit enzyme with a protein density easily detectable by electron microscopy and image processing. Fou...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2000
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15379/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10639128/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.2.617 |
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