Direct localization of a β-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase

To identify the location of a domain of the β-subunit of Escherichia coli RNA polymerase (RNAP) on the three-dimensional structure, we developed a method to tag a nonessential surface of the multisubunit enzyme with a protein density easily detectable by electron microscopy and image processing. Fou...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Opalka, Natacha, Mooney, Rachel A., Richter, Catherine, Severinov, Konstantin, Landick, Robert, Darst, Seth A.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 2000
Schlagworte:	Biological Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15379/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10639128/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.2.617
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