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Direct localization of a β-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase
To identify the location of a domain of the β-subunit of Escherichia coli RNA polymerase (RNAP) on the three-dimensional structure, we developed a method to tag a nonessential surface of the multisubunit enzyme with a protein density easily detectable by electron microscopy and image processing. Fou...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The National Academy of Sciences
2000
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC15379/ https://ncbi.nlm.nih.gov/pubmed/10639128 |
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