Rapid RNA polymerase genetics: one-day, no-column preparation of reconstituted recombinant Escherichia coli RNA polymerase.

We present a simple, rapid procedure for reconstitution of Escherichia coli RNA polymerase holoenzyme (RNAP) from individual recombinant alpha, beta, beta', and sigma 70 subunits. Hexahistidine-tagged recombinant alpha subunit purified by batch-mode metal-ion-affinity chromatography is incubated wit...

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Bibliografske podrobnosti

izdano v:	Proc Natl Acad Sci U S A
Principais autores:	Tang, H, Severinov, K, Goldfarb, A, Ebright, R H
Format:	Artigo
Jezik:	Inglês
Izdano:	National Academy of Sciences 1995
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41815/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7761421/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.11.4902
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