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Inversion of in situ synthesized oligonucleotides: improved reagents for hybridization and primer extension in DNA microarrays.

Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support before the...

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Udgivet i:Nucleic Acids Res
Principais autores: Kwiatkowski, M, Fredriksson, S, Isaksson, A, Nilsson, M, Landegren, U
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1999
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148770/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572170/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.24.4710
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