Inversion of in situ synthesized oligonucleotides: improved reagents for hybridization and primer extension in DNA microarrays.

Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support befor...

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Detalles Bibliográficos
Autores principales: Kwiatkowski, M, Fredriksson, S, Isaksson, A, Nilsson, M, Landegren, U
Formato: Artigo
Lenguaje:Inglês
Publicado: 1999
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC148770/
https://ncbi.nlm.nih.gov/pubmed/10572170
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