Inversion of in situ synthesized oligonucleotides: improved reagents for hybridization and primer extension in DNA microarrays.
Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support before the...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148770/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572170/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.24.4710 |
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