Inversion of in situ synthesized oligonucleotides: improved reagents for hybridization and primer extension in DNA microarrays.

Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support before the...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Kwiatkowski, M, Fredriksson, S, Isaksson, A, Nilsson, M, Landegren, U
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1999
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148770/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572170/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.24.4710
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