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Inversion of in situ synthesized oligonucleotides: improved reagents for hybridization and primer extension in DNA microarrays.
Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support befor...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1999
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC148770/ https://ncbi.nlm.nih.gov/pubmed/10572170 |
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