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Solid-phase synthesis of chelate-labelled oligonucleotides: application in triple-color ligase-mediated gene analysis.

Oligonucleotides labelled with detectable groups are essential tools in gene detection. We describe here the synthesis of pyrimidine deoxynucleotide-building blocks, modified at their C-5 position with a protected form of a strongly chelating agent. These reagents can be used to introduce multiple m...

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Detalhes bibliográficos
Main Authors: Kwiatkowski, M, Samiotaki, M, Lamminmäki, U, Mukkala, V M, Landegren, U
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC308216/
https://ncbi.nlm.nih.gov/pubmed/8041624
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