Nucleotide analogs facilitate base conversion with 3' mismatch primers.
We compared the efficiency of PCR amplification using primers containing either a nucleotide analog or a mismatch at the 3' base. To determine the distribution of bases inserted opposite eight different analogs, 3' analog primers were used to amplify four different templates. The products from the r...
Saved in:
| Published in: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1999
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148388/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10101188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.8.1810 |
| Tags: |
No Tags, Be the first to tag this record!
|