Nucleotide analogs facilitate base conversion with 3' mismatch primers.
We compared the efficiency of PCR amplification using primers containing either a nucleotide analog or a mismatch at the 3' base. To determine the distribution of bases inserted opposite eight different analogs, 3' analog primers were used to amplify four different templates. The products from the r...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148388/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10101188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.8.1810 |
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