Nucleotide analogs facilitate base conversion with 3' mismatch primers.

We compared the efficiency of PCR amplification using primers containing either a nucleotide analog or a mismatch at the 3' base. To determine the distribution of bases inserted opposite eight different analogs, 3' analog primers were used to amplify four different templates. The products from the r...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Day, J P, Bergstrom, D, Hammer, R P, Barany, F
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1999
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148388/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10101188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.8.1810
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