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Nucleotide analogs facilitate base conversion with 3' mismatch primers.

We compared the efficiency of PCR amplification using primers containing either a nucleotide analog or a mismatch at the 3' base. To determine the distribution of bases inserted opposite eight different analogs, 3' analog primers were used to amplify four different templates. The products from the r...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Principais autores: Day, J P, Bergstrom, D, Hammer, R P, Barany, F
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1999
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148388/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10101188/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.8.1810
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