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Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation.

We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to dire...

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Sonraí Bibleagrafaíochta
Main Authors: Fehse, B, Kühlcke, K, Langer, A, Ostertag, W, Lother, H
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1999
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC148236/
https://ncbi.nlm.nih.gov/pubmed/9863001
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