Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation.
We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to dire...
Uloženo v:
| Vydáno v: | Nucleic Acids Res |
|---|---|
| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1999
|
| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9863001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.706 |
| Tagy: |
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|