Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation.
We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to dire...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1999
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9863001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.706 |
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