Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation.

We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to dire...

Descrición completa

Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Fehse, B, Kühlcke, K, Langer, A, Ostertag, W, Lother, H
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1999
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9863001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.706
Tags:	Engadir etiqueta Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!