Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation.

We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to dire...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Fehse, B, Kühlcke, K, Langer, A, Ostertag, W, Lother, H
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1999
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9863001/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.706
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