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OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments

Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method....

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Liu, Chang-Jun, Jiang, Hui, Wu, Lei, Zhu, Ling-Yun, Meng, Er, Zhang, Dong-Yi
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5353728/
https://ncbi.nlm.nih.gov/pubmed/28300166
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep44648
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