Mega primer-mediated molecular cloning strategy for chimaeragenesis and long DNA fragment insertion

Molecular cloning methods based on primer and overlap-extension PCR are widely used due to their simplicity, reliability, low cost and high efficiency. In this article, an efficient mega primer-mediated (MP) cloning strategy for chimaeragenesis and long DNA fragment insertion is presented. MP clonin...

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Bibliographische Detailangaben
Veröffentlicht in:Biosci Rep
Hauptverfasser: Zhang, Hui, Liu, Chang-Jun, Jiang, Hui, Zhou, Lu, Li, Wen-Ying, Zhu, Ling-Yun, Wu, Lei, Meng, Er, Zhang, Dong-Yi
Format: Artigo
Sprache:Inglês
Veröffentlicht: Portland Press Ltd. 2017
Schlagworte:
Research Articles
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5333777/
https://ncbi.nlm.nih.gov/pubmed/28183872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BSR20160608
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