Llwytho...
Restriction-based Multiple-fragment Assembly Strategy to Avoid Random Mutation during Long cDNA Cloning
Long fragment cloning is a challenge for its difficulty in accurate amplifying and tendency to get unwanted mutation. Here we discuss Restriction-based Multiple-fragment Assembly Strategy's advantages and limitations. In this strategy, rather than PCR amplifying the entire coding sequence (CDS)...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | J Cancer |
|---|---|
| Prif Awduron: | , , , , , , , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
Ivyspring International Publisher
2015
|
| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4466412/ https://ncbi.nlm.nih.gov/pubmed/26078793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7150/jca.11779 |
| Tagiau: |
Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
|