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Restriction-based Multiple-fragment Assembly Strategy to Avoid Random Mutation during Long cDNA Cloning
Long fragment cloning is a challenge for its difficulty in accurate amplifying and tendency to get unwanted mutation. Here we discuss Restriction-based Multiple-fragment Assembly Strategy's advantages and limitations. In this strategy, rather than PCR amplifying the entire coding sequence (CDS)...
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| Yayımlandı: | J Cancer |
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| Asıl Yazarlar: | , , , , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Ivyspring International Publisher
2015
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4466412/ https://ncbi.nlm.nih.gov/pubmed/26078793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7150/jca.11779 |
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