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OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments
Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method....
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| Publicat a: | Sci Rep |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5353728/ https://ncbi.nlm.nih.gov/pubmed/28300166 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep44648 |
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