Rapid and efficient cosmid cloning.
We present a procedure for cosmid cloning that allows rapid and efficient cloning of individual DNA fragments of between 32kb and 45kb. By appropriate treatment of the cloning vector, pJb8, we make left-hand and right-hand vector ends that are incapable of self-ligation but which accept dephosporyla...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1981
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327326/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6269067/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.13.2989 |
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