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Rapid and efficient cosmid cloning.

We present a procedure for cosmid cloning that allows rapid and efficient cloning of individual DNA fragments of between 32kb and 45kb. By appropriate treatment of the cloning vector, pJb8, we make left-hand and right-hand vector ends that are incapable of self-ligation but which accept dephosporyla...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Ish-Horowicz, D, Burke, J F
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1981
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327326/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6269067/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.13.2989
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