Fidelity of DNA ligation: a novel experimental approach based on the polymerisation of libraries of oligonucleotides.

Complete libraries of oligonucleotides were used as substrates for Thermus thermophilus DNA ligase, on a M13mp18 ssDNA template. A 17mer primer was used to start a polymerisation process. Ladders of ligation products were analysed by gel electrophoresis. Octa-, nona- and decanucleotide libraries wer...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Housby, J N, Southern, E M
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1998
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9722647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.18.4259
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