Fidelity of DNA ligation: a novel experimental approach based on the polymerisation of libraries of oligonucleotides.
Complete libraries of oligonucleotides were used as substrates for Thermus thermophilus DNA ligase, on a M13mp18 ssDNA template. A 17mer primer was used to start a polymerisation process. Ladders of ligation products were analysed by gel electrophoresis. Octa-, nona- and decanucleotide libraries wer...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9722647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.18.4259 |
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