Fidelity of DNA ligation: a novel experimental approach based on the polymerisation of libraries of oligonucleotides.

Complete libraries of oligonucleotides were used as substrates for Thermus thermophilus DNA ligase, on a M13mp18 ssDNA template. A 17mer primer was used to start a polymerisation process. Ladders of ligation products were analysed by gel electrophoresis. Octa-, nona- and decanucleotide libraries wer...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Housby, J N, Southern, E M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1998
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9722647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.18.4259
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