Fidelity of DNA ligation: a novel experimental approach based on the polymerisation of libraries of oligonucleotides.
Complete libraries of oligonucleotides were used as substrates for Thermus thermophilus DNA ligase, on a M13mp18 ssDNA template. A 17mer primer was used to start a polymerisation process. Ladders of ligation products were analysed by gel electrophoresis. Octa-, nona- and decanucleotide libraries wer...
Sábháilte in:
| Foilsithe in: | Nucleic Acids Res |
|---|---|
| Príomhchruthaitheoirí: | , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe / Cruthaithe: |
Oxford University Press
1998
|
| Ábhair: | |
| Rochtain ar líne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9722647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.18.4259 |
| Clibeanna: |
Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!
|