Fidelity of DNA ligation: a novel experimental approach based on the polymerisation of libraries of oligonucleotides.
Complete libraries of oligonucleotides were used as substrates for Thermus thermophilus DNA ligase, on a M13mp18 ssDNA template. A 17mer primer was used to start a polymerisation process. Ladders of ligation products were analysed by gel electrophoresis. Octa-, nona- and decanucleotide libraries wer...
সংরক্ষণ করুন:
| প্রকাশিত: | Nucleic Acids Res |
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| প্রধান লেখক: | , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
Oxford University Press
1998
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9722647/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.18.4259 |
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