Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...

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Detaylı Bibliyografya
Asıl Yazarlar: Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2002
Konular:
NAR Methods Online
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC137154/
https://ncbi.nlm.nih.gov/pubmed/12384612
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