Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2002
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137154/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12384612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf109
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