Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
פורמט: Artigo
שפה:Inglês
יצא לאור: Oxford University Press 2002
נושאים:
NAR Methods Online
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC137154/
https://ncbi.nlm.nih.gov/pubmed/12384612
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