Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...

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Manylion Llyfryddiaeth

Cyhoeddwyd yn:	Nucleic Acids Res
Prif Awduron:	Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Oxford University Press 2002
Pynciau:	NAR Methods Online
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137154/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12384612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf109
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