Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...

詳細記述

書誌詳細

出版年:	Nucleic Acids Res
主要な著者:	Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
フォーマット:	Artigo
言語:	Inglês
出版事項:	Oxford University Press 2002
主題:	NAR Methods Online
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137154/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12384612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf109
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