Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR
Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...
محفوظ في:
| الحاوية / القاعدة: | Nucleic Acids Res |
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| المؤلفون الرئيسيون: | , , , , , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
Oxford University Press
2002
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137154/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12384612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf109 |
| الوسوم: |
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