Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

Reverse genetic approaches to understanding gene function would be greatly facilitated by increasing the efficiency of methods for isolating mutants without the reliance on a predicted phenotype. Established PCR-based methods of isolating deletion mutants are widely used for this purpose in Caenorha...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2002
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137154/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12384612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf109
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