RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemi...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Yu, Jenn-Yah, DeRuiter, Stacy L., Turner, David L.
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 2002
Assuntos:	Biological Sciences
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122899/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972060/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.092143499
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