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RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemi...

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Hlavní autoři: Yu, Jenn-Yah, DeRuiter, Stacy L., Turner, David L.
Médium: Artigo
Jazyk:Inglês
Vydáno: The National Academy of Sciences 2002
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC122899/
https://ncbi.nlm.nih.gov/pubmed/11972060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.092143499
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