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RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemi...

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書目詳細資料
Main Authors: Yu, Jenn-Yah, DeRuiter, Stacy L., Turner, David L.
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences 2002
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC122899/
https://ncbi.nlm.nih.gov/pubmed/11972060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.092143499
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