RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemi...

Celý popis

Podrobná bibliografie

Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Yu, Jenn-Yah, DeRuiter, Stacy L., Turner, David L.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 2002
Témata:	Biological Sciences
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122899/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972060/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.092143499
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!