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RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells
Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemi...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The National Academy of Sciences
2002
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC122899/ https://ncbi.nlm.nih.gov/pubmed/11972060 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.092143499 |
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