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Mutagenesis by reversible promoter insertion to study the activation of NF-κB

Genetic dissection of signaling pathways in mammalian cells involves screening or selecting phenotypic mutants obtained by a variety of techniques. Limitations in current methods include inadequate genome coverage and difficulty in validating the link between mutation and phenotype. We describe an i...

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Detalhes bibliográficos
Main Authors: Kandel, Eugene S., Lu, Tao, Wan, Youzhong, Agarwal, Mukesh K., Jackson, Mark W., Stark, George R.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1088393/
https://ncbi.nlm.nih.gov/pubmed/15851657
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0502463102
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