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3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3′-MGB probes was explored. In comparison with unmodified DNA, MGB pr...

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Gorde:
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Kutyavin, Igor V., Afonina, Irina A., Mills, Alan, Gorn, Vladimir V., Lukhtanov, Eugeny A., Belousov, Evgeniy S., Singer, Michael J., Walburger, David K., Lokhov, Sergey G., Gall, Alexander A., Dempcy, Robert, Reed, Michael W., Meyer, Rich B., Hedgpeth, Joe
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2000
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102528/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10606668/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.2.655
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