3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3′-MGB probes was explored. In comparison with unmodified DNA, MGB pr...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Kutyavin, Igor V., Afonina, Irina A., Mills, Alan, Gorn, Vladimir V., Lukhtanov, Eugeny A., Belousov, Evgeniy S., Singer, Michael J., Walburger, David K., Lokhov, Sergey G., Gall, Alexander A., Dempcy, Robert, Reed, Michael W., Meyer, Rich B., Hedgpeth, Joe
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2000
Témata:	Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102528/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10606668/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.2.655
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