3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3′-MGB probes was explored. In comparison with unmodified DNA, MGB pr...

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Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Kutyavin, Igor V., Afonina, Irina A., Mills, Alan, Gorn, Vladimir V., Lukhtanov, Eugeny A., Belousov, Evgeniy S., Singer, Michael J., Walburger, David K., Lokhov, Sergey G., Gall, Alexander A., Dempcy, Robert, Reed, Michael W., Meyer, Rich B., Hedgpeth, Joe
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2000
Предметы:	Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102528/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10606668/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.2.655
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