3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3′-MGB probes was explored. In comparison with unmodified DNA, MGB pr...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Kutyavin, Igor V., Afonina, Irina A., Mills, Alan, Gorn, Vladimir V., Lukhtanov, Eugeny A., Belousov, Evgeniy S., Singer, Michael J., Walburger, David K., Lokhov, Sergey G., Gall, Alexander A., Dempcy, Robert, Reed, Michael W., Meyer, Rich B., Hedgpeth, Joe
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2000
Assuntos:	Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102528/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10606668/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.2.655
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!