3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3′-MGB probes was explored. In comparison with unmodified DNA, MGB pr...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Hoofdauteurs: Kutyavin, Igor V., Afonina, Irina A., Mills, Alan, Gorn, Vladimir V., Lukhtanov, Eugeny A., Belousov, Evgeniy S., Singer, Michael J., Walburger, David K., Lokhov, Sergey G., Gall, Alexander A., Dempcy, Robert, Reed, Michael W., Meyer, Rich B., Hedgpeth, Joe
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2000
Onderwerpen:
Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC102528/
https://ncbi.nlm.nih.gov/pubmed/10606668
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items