Activity-based Pull-down of Proteolytic Standard and Immunoproteasome Subunits
Activity-based probes (ABP) are small organic molecules that irreversibly bind to the active center of a specific enzyme family and may be coupled to a fluorophore or an affinity tag (Li et al., 2013). Here, we describe a method to pull-down active catalytic standard and immunoproteasome subunits in...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Bio-protocol LLC
2016-12-01
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| Serie: | Bio-Protocol |
| Accesso online: | https://bio-protocol.org/en/bpdetail?id=2073&type=0 |
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