Activity-based Pull-down of Proteolytic Standard and Immunoproteasome Subunits

Activity-based probes (ABP) are small organic molecules that irreversibly bind to the active center of a specific enzyme family and may be coupled to a fluorophore or an affinity tag (Li et al., 2013). Here, we describe a method to pull-down active catalytic standard and immunoproteasome subunits in...

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Hlavní autoři:	Tobias Baumann, Oliver Vosyka, Bogdan Florea, Hermen Overkleeft, Silke Meiners, Ilona Kammerl
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Bio-protocol LLC 2016-12-01
Edice:	Bio-Protocol
On-line přístup:	https://bio-protocol.org/en/bpdetail?id=2073&type=0
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