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Activity-based Pull-down of Proteolytic Standard and Immunoproteasome Subunits

Activity-based probes (ABP) are small organic molecules that irreversibly bind to the active center of a specific enzyme family and may be coupled to a fluorophore or an affinity tag (Li et al., 2013). Here, we describe a method to pull-down active catalytic standard and immunoproteasome subunits in...

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Váldodahkkit: Tobias Baumann, Oliver Vosyka, Bogdan Florea, Hermen Overkleeft, Silke Meiners, Ilona Kammerl
Materiálatiipa: Artigo
Giella:Inglês
Almmustuhtton: Bio-protocol LLC 2016-12-01
Ráidu:Bio-Protocol
Liŋkkat:https://bio-protocol.org/en/bpdetail?id=2073&type=0
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