Purification of Anthrax Edema Factor from Escherichia coli and Identification of Residues Required for Binding to Anthrax Protective Antigen
The structural gene for anthrax edema factor (EF) was expressed in Escherichia coli under the control of a powerful T5 promoter to yield the 89-kDa recombinant protein that reacted with anti-EF antibodies. Recombinant EF was purified to homogeneity by a two-step procedure involving metal chelate aff...
Guardat en:
| Publicat a: | Infect Immun |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
2001
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC98792/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11553601/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.69.10.6532-6536.2001 |
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