Purification of Anthrax Edema Factor from Escherichia coli and Identification of Residues Required for Binding to Anthrax Protective Antigen

The structural gene for anthrax edema factor (EF) was expressed in Escherichia coli under the control of a powerful T5 promoter to yield the 89-kDa recombinant protein that reacted with anti-EF antibodies. Recombinant EF was purified to homogeneity by a two-step procedure involving metal chelate aff...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Kumar, Praveen, Ahuja, Nidhi, Bhatnagar, Rakesh
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Molecular Pathogenesis
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC98792/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11553601/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.69.10.6532-6536.2001
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