Purification of Anthrax Edema Factor from Escherichia coli and Identification of Residues Required for Binding to Anthrax Protective Antigen

The structural gene for anthrax edema factor (EF) was expressed in Escherichia coli under the control of a powerful T5 promoter to yield the 89-kDa recombinant protein that reacted with anti-EF antibodies. Recombinant EF was purified to homogeneity by a two-step procedure involving metal chelate aff...

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Bibliografiske detaljer
Main Authors: Kumar, Praveen, Ahuja, Nidhi, Bhatnagar, Rakesh
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Molecular Pathogenesis
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC98792/
https://ncbi.nlm.nih.gov/pubmed/11553601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.69.10.6532-6536.2001
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